|
KMID : 1141520220370030552
|
|
Endocrinology and Metabolism
2022 Volume.37 No. 3 p.552 ~ p.557
|
|
|
Sestrin2 Regulates Beneficial β3-Adrenergic Receptor-Mediated Effects Observed in Inguinal White Adipose Tissue and Soleus Muscle
|
|
Park Min-Jeong
Kim Joo-Won
Roh Eun
Choi Kyung-Mook
Baik Sei-Hyun
Hwang Hwan-Jin
Yoo Hye-Jin
|
|
|
Abstract
|
|
|
|
Sestrin2, a well-known adenosine monophosphate-activated protein kinase (AMPK) regulator, plays a protective role against metabolic stress. The β3-adrenergic receptor (β3AR) induces fat browning and inhibits muscle atrophy in an AMPK-dependent manner. However, no prior research has examined the relationship of sestrin2 with β3AR in body composition changes. In this study, CL 316,243 (CL), a β3AR agonist, was administered to wild-type and sestrin2-knockout (KO) mice for 2 weeks, and fat and muscle tissues were harvested. CL induced AMPK phosphorylation, expression of brown-fat markers, and mitochondrial biogenesis, which resulted in the reduction of lipid droplet size in inguinal white adipose tissue (iWAT). These effects were not observed in sestrin2-KO mice. In CL-treated soleus muscle, sestrin2-KO was related to decreased myogenic gene expression and increased levels of muscle atrophy-related molecules. Our results suggest that sestrin2 is associated with beneficial β3AR-mediated changes in body composition, especially in iWAT and in the soleus.
|
|
|
KEYWORD
|
|
|
Sestrin2 protein, mouse, Adrenergic beta-3 receptor agonists, Adipose tissue, brown, Muscle development, Muscular atrophy
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
    
|
|